› 8pej

X-ray diffraction
1.5Å resolution

CjGH35 with a Galactosidase Activity-Based Probe

Released:
DOI: 10.2210/pdb8pej/pdb
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PDB entry 8pej coloured by chain, front view.
PDB entry 8pej coloured by chain, side view.
PDB entry 8pej coloured by chain, top view.
Source organism: Cellvibrio japonicus Ueda107
Primary publication:
The development of a broad-spectrum retaining β-exo-galactosidase activity-based probe.
Kuo CL, Su Q, van den Nieuwendijk AMCH, Beenakker TJM, Offen WA, Willems LI, Boot RG, Sarris AJ, Marques ARA, Codée JDC, van der Marel GA, Florea BI, Davies GJ, Overkleeft HS, Aerts JMFG
Org Biomol Chem (2023)
PMID: 37724332

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109884 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase, putative, bgl35A Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 550 amino acids
Theoretical weight: 62.13 KDa
Source organism: Cellvibrio japonicus Ueda107
Expression system: Escherichia coli
UniProt:
  • Canonical: B3PBE0 (Residues: 36-575; Coverage: 100%)
Gene names: CJA_2707, bgl35A
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand NA 35 x NA
Ligand YGX 8 x YGX
Ligand ACT 14 x ACT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 99.316Å b: 116.212Å c: 116.396Å
α: 90.236° β: 89.889° γ: 90.094°
R-values:
R R work R free
0.158 0.157 0.181
Expression system: Escherichia coli

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Citations

1 review citation

From Mechanism-Based Retaining Glycosidase Inhibitors to Activity-Based Glycosidase Profiling.
Artola et al. (2024)
1 other article

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