› 8pfc

X-ray diffraction
2.2Å resolution

Crystal structure of binary complex between Aster yellows witches'-broom phytoplasma effector SAP05 and the zinc finger domain of SPL5 from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb8pfc/pdb
PDB entry 8pfc coloured by chain, front view.
PDB entry 8pfc coloured by chain, side view.
PDB entry 8pfc coloured by chain, top view.
Source organisms:
Primary publication:
Bimodular architecture of bacterial effector SAP05 that drives ubiquitin-independent targeted protein degradation.
Liu Q, Maqbool A, Mirkin FG, Singh Y, Stevenson CEM, Lawson DM, Kamoun S, Huang W, Hogenhout SA
Proc Natl Acad Sci U S A 120 e2310664120 (2023)
PMID: 38039272

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-220948 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sequence-variable mosaic (SVM) signal sequence domain-containing protein Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 105 amino acids
Theoretical weight: 12.31 KDa
Source organism: Aster yellows witches'-broom phytoplasma AYWB
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2NK94 (Residues: 33-135; Coverage: 100%)
Gene name: AYWB_032
Squamosa promoter-binding-like protein 5 Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 70 amino acids
Theoretical weight: 8.23 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9S758 (Residues: 59-127; Coverage: 38%)
Gene names: At3g15270, K7L4.7, SPL5
Sequence domains: SBP domain

Ligands and Environments

1 bound ligand:
Ligand ZN 20 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 78.686Å b: 165.019Å c: 80.862Å
α: 90° β: 109.65° γ: 90°
R-values:
R R work R free
0.224 0.223 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Bimodular architecture of bacterial effector SAP05 that drives ubiquitin-independent targeted protein degradation.
Liu et al. (2023)