› 8pia

X-ray diffraction
2.8Å resolution

DNA binding domain of HNF-1A bound to P2-HNF4A promoter DNA variant (P2 -181G>T)

Released:
DOI: 10.2210/pdb8pia/pdb
PDB entry 8pia coloured by chain, front view.
PDB entry 8pia coloured by chain, side view.
PDB entry 8pia coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular mechanism of HNF-1A-mediated HNF4A gene regulation and promoter-driven HNF4A-MODY diabetes.
Kind L, Molnes J, Tjora E, Raasakka A, Myllykoski M, Colclough K, Saint-Martin C, Adelfalk C, Dusatkova P, Pruhova S, Valtonen-André C, Bellanné-Chantelot C, Arnesen T, Kursula P, Njølstad PR
JCI Insight 9 (2024)
PMID: 38855865

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245989 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Hepatocyte nuclear factor 1-alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 198 amino acids
Theoretical weight: 22.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20823 (Residues: 83-279; Coverage: 31%)
Gene names: HNF1A, TCF1
Sequence domains: Hepatocyte nuclear factor 1 (HNF-1), N terminus
Chains: E Chain: E
Molecule details ›
Chain: E
Length: 21 nucleotides
Theoretical weight: 6.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
Chains: F Chain: F
Molecule details ›
Chain: F
Length: 21 nucleotides
Theoretical weight: 6.5 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P212121
Unit cell:
a: 48.695Å b: 48.752Å c: 215.811Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.245 0.3
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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