8pil

Electron Microscopy
3.2Å resolution

E. coli transcription complex paused at ops site and bound to RfaH and NusA

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-243367 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (9 distinct):
Transcription antitermination protein RfaH Chain: P
Molecule details ›
Chain: P
Length: 164 amino acids
Theoretical weight: 18.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFW0 (Residues: 1-162; Coverage: 100%)
Gene names: JW3818, b3842, hlyT, rfaH, sfrB
Sequence domains: Transcription termination factor nusG
DNA-directed RNA polymerase subunit beta Chain: I
Molecule details ›
Chain: I
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: J
DNA-directed RNA polymerase subunit omega Chain: K
DNA-directed RNA polymerase subunit alpha Chains: G, H
Transcription termination/antitermination protein NusA Chain: F
non-template DNA Chain: A
template DNA Chain: B
RNA Chain: R

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-17685
Expression system: Escherichia coli BL21(DE3)