› 8piu

Electron Microscopy
2.9Å resolution

60-meric complex of dihydrolipoamide acetyltransferase (E2) of the human pyruvate dehydrogenase complex

Released:
DOI: 10.2210/pdb8piu/pdb
PDB entry 8piu coloured by chain, front view.
PDB entry 8piu coloured by chain, side view.
PDB entry 8piu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Stoichiometry and architecture of the human pyruvate dehydrogenase complex.
Zdanowicz R, Afanasyev P, Pruška A, Harrison JA, Giese C, Boehringer D, Leitner A, Zenobi R, Glockshuber R
Sci Adv 10 eadn4582 (2024)
PMID: 39018392
Related structures: EMD-17691

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo 60-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145330 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial Chains: A, AA, AB, B, BA, BB, C, CA, CB, D, DA, DB, E, EA, EB, F, FA, FB, G, GA, GB, H, HA, HB, I, IA, IB, J, JA, K, KA, L, LA, M, MA, N, NA, O, OA, P, PA, Q, QA, R, RA, S, SA, T, TA, UA, V, VA, W, WA, X, XA, Y, YA, Z, ZA
Molecule details ›
Chains: A, AA, AB, B, BA, BB, C, CA, CB, D, DA, DB, E, EA, EB, F, FA, FB, G, GA, GB, H, HA, HB, I, IA, IB, J, JA, K, KA, L, LA, M, MA, N, NA, O, OA, P, PA, Q, QA, R, RA, S, SA, T, TA, UA, V, VA, W, WA, X, XA, Y, YA, Z, ZA
Length: 562 amino acids
Theoretical weight: 59.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10515 (Residues: 86-647; Coverage: 87%)
Gene names: DLAT, DLTA
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-17691
Expression system: Escherichia coli BL21(DE3)

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