8pj1

Electron Microscopy
3.4Å resolution

Structure of human 48S translation initiation complex in open codon scanning state (48S-1)

Released:
Source organism: Homo sapiens
Related structures: EMD-17696

Function and Biology Details

Reactions catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 54-mer (preferred)
PDBe Complex ID:
PDB-CPX-249646 (preferred)
Entry contents:
51 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (54 distinct):
Eukaryotic translation initiation factor 3 subunit B Chain: 1
Molecule details ›
Chain: 1
Length: 814 amino acids
Theoretical weight: 92.59 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P55884 (Residues: 1-814; Coverage: 100%)
Gene names: EIF3B, EIF3S9
Sequence domains:
Eukaryotic translation initiation factor 3 subunit I Chain: 2
Molecule details ›
Chain: 2
Length: 325 amino acids
Theoretical weight: 36.54 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q13347 (Residues: 1-325; Coverage: 100%)
Gene names: EIF3I, EIF3S2, TRIP1
Sequence domains:
Eukaryotic translation initiation factor 3 subunit K Chain: 3
Eukaryotic translation initiation factor 3 subunit F Chain: 4
Eukaryotic translation initiation factor 3 subunit L Chain: 5
Eukaryotic translation initiation factor 3 subunit M Chain: 6
Eukaryotic translation initiation factor 3 subunit H Chain: 8
Small ribosomal subunit protein eS32 Chain: 9
Small ribosomal subunit protein uS17 Chain: B
Small ribosomal subunit protein eS4, X isoform Chain: C
Small ribosomal subunit protein uS4 Chain: D
Small ribosomal subunit protein uS12 Chain: E
Small ribosomal subunit protein eS30 Chain: F
Small ribosomal subunit protein eS7 Chain: G
Small ribosomal subunit protein eS27 Chain: H
Small ribosomal subunit protein uS15 Chain: I
Small ribosomal subunit protein uS8 Chain: J
Small ribosomal subunit protein eS21 Chain: K
Small ribosomal subunit protein uS5 Chain: L
Small ribosomal subunit protein eS17 Chain: M
Small ribosomal subunit protein uS2 Chain: N
Small ribosomal subunit protein eS1 Chain: O
Small ribosomal subunit protein uS11 Chain: P
Small ribosomal subunit protein eS26 Chain: Q
Small ribosomal subunit protein eS8 Chain: R
Small ribosomal subunit protein eS6 Chain: S
Small ribosomal subunit protein eS24 Chain: T
Small ribosomal subunit protein uS7 Chain: V
Small ribosomal subunit protein uS9 Chain: Y
Small ribosomal subunit protein uS3 Chain: Z
Small ribosomal subunit protein eS10 Chain: a
Small ribosomal subunit protein uS19 Chain: b
Small ribosomal subunit protein RACK1 Chain: c
Small ribosomal subunit protein eS19 Chain: d
Small ribosomal subunit protein eS25 Chain: e
Small ribosomal subunit protein uS13 Chain: f
Small ribosomal subunit protein uS10 Chain: h
Small ribosomal subunit protein uS14 Chain: i
Ubiquitin-ribosomal protein eS31 fusion protein Chain: k
Small ribosomal subunit protein eS12 Chain: m
Small ribosomal subunit protein eS28 Chain: n
Eukaryotic translation initiation factor 3 subunit G Chain: o
Eukaryotic translation initiation factor 1 Chain: p
Eukaryotic translation initiation factor 1A, X-chromosomal Chain: q
Eukaryotic translation initiation factor 2 subunit 1 Chain: r
Eukaryotic translation initiation factor 2 subunit 2 Chain: s
Eukaryotic translation initiation factor 2 subunit 3 Chain: t
Eukaryotic translation initiation factor 3 subunit A Chain: u
Eukaryotic translation initiation factor 3 subunit E Chain: v
Eukaryotic translation initiation factor 3 subunit D Chain: x
Eukaryotic translation initiation factor 3 subunit C Chain: y
Messenger RNA Chain: 7
18S Ribosomal RNA Chain: A
Initiator Met-tRNA-i Chain: w

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-17696
Expression system: Escherichia coli