› 8pk1

Electron Microscopy
3.17Å resolution

Cas1-Cas2 CRISPR integrase bound to prespacer DNA, Streptococcus thermophilus DGCC 7710 CRISPR3 system

Released:
DOI: 10.2210/pdb8pk1/pdb
PDB entry 8pk1 coloured by chain, front view.
PDB entry 8pk1 coloured by chain, side view.
PDB entry 8pk1 coloured by chain, top view.
Source organism: Streptococcus thermophilus DGCC 7710
Entry authors: Sasnauskas G, Gaizauskaite U, Tamulaitiene G
Related structures: EMD-17722

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270652 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
CRISPR-associated endoribonuclease Cas2 Chains: A, D
Molecule details ›
Chains: A, D
Length: 114 amino acids
Theoretical weight: 13.43 KDa
Source organism: Streptococcus thermophilus DGCC 7710
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3ECR3 (Residues: 1-114; Coverage: 100%)
Gene name: cas2
Sequence domains: CRISPR associated protein Cas2
CRISPR-associated endonuclease Cas1 Chains: B, C, E, F
Molecule details ›
Chains: B, C, E, F
Length: 302 amino acids
Theoretical weight: 35.36 KDa
Source organism: Streptococcus thermophilus DGCC 7710
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3ECR2 (Residues: 1-289; Coverage: 100%)
Gene name: cas1
Sequence domains: CRISPR associated protein Cas1
Chains: G,J Chains: G, J
Molecule details ›
Chains: G, J
Length: 26 nucleotides
Theoretical weight: 7.92 KDa
Chains: H,K Chains: H, K
Molecule details ›
Chains: H, K
Length: 26 nucleotides
Theoretical weight: 8.06 KDa

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.17Å
Relevant EMDB volumes: EMD-17722
Expression system: Escherichia coli BL21(DE3)

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