› 8pk8

Electron Microscopy
2.49Å resolution

Structure of the human mitochondrial iron-sulfur cluster biosynthesis complex during persulfide transfer (persulfide on ISCU2)

Released:
DOI: 10.2210/pdb8pk8/pdb
PDB entry 8pk8 coloured by chain, front view.
PDB entry 8pk8 coloured by chain, side view.
PDB entry 8pk8 coloured by chain, top view.
Source organisms:
Primary publication:
Mechanism and structural dynamics of sulfur transfer during de novo [2Fe-2S] cluster assembly on ISCU2.
Schulz V, Steinhilper R, Oltmanns J, Freibert SA, Krapoth N, Linne U, Welsch S, Hoock MH, Schünemann V, Murphy BJ, Lill R
OpenAccess logo Nat Commun 15 3269 (2024)
PMID: 38627381
Related structures: EMD-17732

Function and Biology Details

Reactions catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-243727 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Cysteine desulfurase Chain: A
Molecule details ›
Chain: A
Length: 404 amino acids
Theoretical weight: 44.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y697 (Residues: 56-457; Coverage: 88%)
Gene names: HUSSY-08, NFS1, NIFS
Sequence domains: Aminotransferase class-V
LYR motif-containing protein 4 Chain: B
Molecule details ›
Chain: B
Length: 115 amino acids
Theoretical weight: 13.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HD34 (Residues: 1-91; Coverage: 100%)
Gene names: C6orf149, CGI-203, ISD11, LYRM4
Sequence domains: Complex 1 protein (LYR family)
Acyl carrier protein Chain: C
Molecule details ›
Chain: C
Length: 78 amino acids
Theoretical weight: 8.65 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6A8 (Residues: 1-78; Coverage: 100%)
Gene names: JW1080, acpP, b1094
Sequence domains: Phosphopantetheine attachment site
Iron-sulfur cluster assembly enzyme ISCU Chain: D
Molecule details ›
Chain: D
Length: 143 amino acids
Theoretical weight: 15.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H1K1 (Residues: 35-167; Coverage: 80%)
Gene names: ISCU, NIFUN
Sequence domains: NifU-like N terminal domain
Frataxin mature form Chain: E
Molecule details ›
Chain: E
Length: 133 amino acids
Theoretical weight: 14.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16595 (Residues: 81-210; Coverage: 62%)
Gene names: FRDA, FXN, X25
Sequence domains: Frataxin-like domain

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
2 bound ligands:
Ligand 8Q1 1 x 8Q1
Ligand FE2 1 x FE2
1 modified residue:
Ligand CSS 1 x CSS

Experiments and Validation Details

Entry percentile scores
Resolution: 2.49Å
Relevant EMDB volumes: EMD-17732
Expression system: Escherichia coli BL21(DE3)

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