› 8pkh

Electron Microscopy
3.35Å resolution

Borrelia bacteriophage BB1 procapsid, fivefold-symmetrized outer shell

Released:
DOI: 10.2210/pdb8pkh/pdb
PDB entry 8pkh coloured by chain, front view.
PDB entry 8pkh coloured by chain, side view.
PDB entry 8pkh coloured by chain, top view.
Source organism: Borreliella burgdorferi B31
Primary publication:
Structure of the Borrelia bacteriophage φBB1 procapsid.
Rūmnieks J, Füzik T, Tārs K
J Mol Biol 168323 (2023)
PMID: 37866476
Related structures: EMD-17739

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 1245-mer (preferred)
PDBe Complex ID:
PDB-CPX-236107 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Cytosolic protein Chains: AA, AB, AC, AD, AE, AF, AG, AH, AI, AJ, AK, AL, AM, AN, AO, AP, AQ, AR, AS, AT, AU, AV, AW, AX, AY, AZ, BA, BB, BC, BD, BE, BF, BG, BH, BI, BJ, BK, BL, BM, BN, BO, BP, BQ, BR, BS, BT, BU, BV, BW, BX, BY, BZ, CA, CB, CC, CD, CE, CF, CG, CH, CI, CJ, CK, CL, CM, CN, CO, CP, CQ, CR, CS, CT, CU, CV, CW, CX, CY, CZ, DA, DB, DC, DD, DE
Molecule details ›
Chains: AA, AB, AC, AD, AE, AF, AG, AH, AI, AJ, AK, AL, AM, AN, AO, AP, AQ, AR, AS, AT, AU, AV, AW, AX, AY, AZ, BA, BB, BC, BD, BE, BF, BG, BH, BI, BJ, BK, BL, BM, BN, BO, BP, BQ, BR, BS, BT, BU, BV, BW, BX, BY, BZ, CA, CB, CC, CD, CE, CF, CG, CH, CI, CJ, CK, CL, CM, CN, CO, CP, CQ, CR, CS, CT, CU, CV, CW, CX, CY, CZ, DA, DB, DC, DD, DE
Length: 319 amino acids
Theoretical weight: 36.26 KDa
Source organism: Borreliella burgdorferi B31
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9R3U6 (Residues: 1-319; Coverage: 100%)
Gene names: BB_L06, BB_O06, BB_P06, BB_S06
Decorator protein P03 Chains: DF, DG, DH, DI, DJ, DK, DL, DM, DN, DO, DP, DQ, DR, DS, DT, DU, DV, DW, DX, DY, DZ, EA, EB, EC, ED, EE, EF, EG, EH, EI, EJ, EK, EL, EM, EN, EO, EP, EQ, ER, ES, ET, EU, EV, EW, EX, EY, EZ, FA, FB, FC, FD, FE, FF, FG, FH, FI
Molecule details ›
Chains: DF, DG, DH, DI, DJ, DK, DL, DM, DN, DO, DP, DQ, DR, DS, DT, DU, DV, DW, DX, DY, DZ, EA, EB, EC, ED, EE, EF, EG, EH, EI, EJ, EK, EL, EM, EN, EO, EP, EQ, ER, ES, ET, EU, EV, EW, EX, EY, EZ, FA, FB, FC, FD, FE, FF, FG, FH, FI
Length: 185 amino acids
Theoretical weight: 20.03 KDa
Source organism: Borreliella burgdorferi B31
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9R2Q0 (Residues: 1-185; Coverage: 100%)
Gene names: BB_L03, BB_P03
Sequence domains: Lyme disease proteins of unknown function
Decorator protein P04 Chain: FJ
Molecule details ›
Chain: FJ
Length: 254 amino acids
Theoretical weight: 28.07 KDa
Source organism: Borreliella burgdorferi B31
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9R3E0 (Residues: 1-254; Coverage: 100%)
Gene names: BB_L04, BB_P04
Sequence domains: Lyme disease proteins of unknown function
Decorator protein P05 Chains: FK, FL, FM, FN, FO, FP, FQ, FR, FS, FT, FU, FV, FW, FX, FY, FZ, GA, GB, GC, GD, GE, GF, GG, GH, GI, GJ
Molecule details ›
Chains: FK, FL, FM, FN, FO, FP, FQ, FR, FS, FT, FU, FV, FW, FX, FY, FZ, GA, GB, GC, GD, GE, GF, GG, GH, GI, GJ
Length: 190 amino acids
Theoretical weight: 21.27 KDa
Source organism: Borreliella burgdorferi B31
Expression system: Escherichia coli BL21
Scaffold protein Chains: GK, GL, GM, GN, GO, GP, GQ, GR, GS, GT, GU, GV, GW, GX, GY, GZ, HA, HB, HC, HD, HE, HF, HG, HH, HI, HJ, HK, HL, HM, HN, HO, HP, HQ, HR, HS, HT, HU, HV, HW, HX, HY, HZ, IA, IB, IC, ID, IE, IF, IG, IH, II, IJ, IK, IL, IM, IN, IO, IP, IQ, IR, IS, IT, IU, IV, IW, IX, IY, IZ, JA, JB, JC, JD, JE, JF, JG, JH, JI, JJ, JK, JL, JM, JN, JO
Molecule details ›
Chains: GK, GL, GM, GN, GO, GP, GQ, GR, GS, GT, GU, GV, GW, GX, GY, GZ, HA, HB, HC, HD, HE, HF, HG, HH, HI, HJ, HK, HL, HM, HN, HO, HP, HQ, HR, HS, HT, HU, HV, HW, HX, HY, HZ, IA, IB, IC, ID, IE, IF, IG, IH, II, IJ, IK, IL, IM, IN, IO, IP, IQ, IR, IS, IT, IU, IV, IW, IX, IY, IZ, JA, JB, JC, JD, JE, JF, JG, JH, JI, JJ, JK, JL, JM, JN, JO
Length: 230 amino acids
Theoretical weight: 26.71 KDa
Source organism: Borreliella burgdorferi B31
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9R2Q2 (Residues: 1-230; Coverage: 100%)
Gene names: BB_L02, BB_P02, BB_S02
Sequence domains: Protein of unknown function (DUF1357)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.35Å
Relevant EMDB volumes: EMD-17739
Expression system: Escherichia coli BL21

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