› 8pko

Electron Microscopy
2.6Å resolution

The ERAD misfolded glycoprotein checkpoint complex from Chaetomium thermophilum (EDEM:PDI heterodimer).

Released:
DOI: 10.2210/pdb8pko/pdb
PDB entry 8pko coloured by chain, front view.
PDB entry 8pko coloured by chain, side view.
PDB entry 8pko coloured by chain, top view.
Source organisms:
Entry authors: Roversi P, Hitchman CJ, Lia A, Bayo Y
Related structures: EMD-17749

Function and Biology Details

Reaction catalysed: 
Catalyzes the rearrangement of -S-S- bonds in proteins
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271218 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Green fluorescent protein; alpha-1,2-Mannosidase Chain: A
Molecule details ›
Chain: A
Length: 1284 amino acids
Theoretical weight: 142.16 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P42212 (Residues: 6-238; Coverage: 98%)
  • Canonical: G0SCX7 (Residues: 63-1092; Coverage: 94%)
Gene names: CTHT_0058730, GFP
Sequence domains:
Protein disulfide-isomerase Chain: B
Molecule details ›
Chain: B
Length: 507 amino acids
Theoretical weight: 55.84 KDa
Source organism: Thermochaetoides thermophila
Expression system: Homo sapiens
UniProt:
  • Canonical: G0SGS2 (Residues: 21-515; Coverage: 99%)
Gene name: CTHT_0067360
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand CA 1 x CA
Ligand THJ 1 x THJ
Ligand NAG 2 x NAG
Ligand MAN 1 x MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.6Å
Relevant EMDB volumes: EMD-17749
Expression system: Homo sapiens

Quick links