› 8pm0

Electron Microscopy
2.9Å resolution

Influenza A/H7N9 polymerase in replicase-like conformation in pre-initiation state with Pol II pS5 CTD peptide mimic bound in site 1A/2A

Released:
DOI: 10.2210/pdb8pm0/pdb
PDB entry 8pm0 coloured by chain, front view.
PDB entry 8pm0 coloured by chain, side view.
PDB entry 8pm0 coloured by chain, top view.
Source organisms:
Primary publication:
The host RNA polymerase II C-terminal domain is the anchor for replication of the influenza virus genome.
Krischuns T, Arragain B, Isel C, Paisant S, Budt M, Wolff T, Cusack S, Naffakh N
OpenAccess logo Nat Commun 15 1064 (2024)
PMID: 38316757
Related structures: EMD-17755

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-240169 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 729 amino acids
Theoretical weight: 84.35 KDa
Source organism: Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: M9TI86 (Residues: 1-716; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 757 amino acids
Theoretical weight: 86.5 KDa
Source organism: Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: M9TLW3 (Residues: 1-757; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chain: C
Molecule details ›
Chain: C
Length: 788 amino acids
Theoretical weight: 89.04 KDa
Source organism: Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: X5F427 (Residues: 1-759; Coverage: 100%)
Gene name: PB2
Sequence domains:
RNA Pol II CTD 6 repeats (site 1A/2A) Chains: G, P
Molecule details ›
Chains: G, P
Length: 42 amino acids
Theoretical weight: 4.82 KDa
Source organism: Homo sapiens
Expression system: Not provided
51-mer vRNA loop (v51_mut_S) Chain: V
Molecule details ›
Chain: V
Length: 51 nucleotides
Theoretical weight: 16.09 KDa

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand SEP 12 x SEP

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-17755
Expression systems:
  • Trichoplusia ni
  • Not provided

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