› 8pm4

Electron Microscopy
2.93Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270653 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated DNA-binding protein Cas12m Chain: A
Molecule details ›
Chain: A
Length: 614 amino acids
Theoretical weight: 68.22 KDa
Source organism: Gordonia otitidis NBRC 100426
Expression system: Escherichia coli str. K-12 substr. DH10B
UniProt:
  • Canonical: H5TRP0 (Residues: 1-607; Coverage: 100%)
Gene names: GOOTI_202_00040, cas12m
DNA oligoduplex, target strand, chain C Chain: C
Molecule details ›
Chain: C
Length: 44 nucleotides
Theoretical weight: 13.56 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA oligoduplex, non-target strand, chain D Chain: D
Molecule details ›
Chain: D
Length: 44 nucleotides
Theoretical weight: 13.54 KDa
Source organism: synthetic construct
Expression system: Not provided
crRNA, chain B Chain: B
Molecule details ›
Chain: B
Length: 58 nucleotides
Theoretical weight: 18.75 KDa
Source organism: Gordonia otitidis NBRC 100426
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.93Å
Relevant EMDB volumes: EMD-17757
Expression systems:
  • Escherichia coli str. K-12 substr. DH10B
  • Not provided

Quick links

Citations

1 citations in other articles

Innate programmable DNA binding by CRISPR-Cas12m effectors enable efficient base editing.
Bigelyte et al. (2024)