› 8pnw

X-ray diffraction
1.7Å resolution

Crystal structure of D-amino acid aminotransferase from Blastococcus saxobsidens in holo form with PLP

Released:
DOI: 10.2210/pdb8pnw/pdb
PDB entry 8pnw coloured by chain, front view.
PDB entry 8pnw coloured by chain, side view.
PDB entry 8pnw coloured by chain, top view.
Source organism: Blastococcus saxobsidens
Primary publication:
Expanded Substrate Specificity in D-Amino Acid Transaminases: A Case Study of Transaminase from Blastococcus saxobsidens.
Shilova SA, Matyuta IO, Petrova ES, Nikolaeva AY, Rakitina TV, Minyaev ME, Boyko KM, Popov VO, Bezsudnova EY
Int J Mol Sci 24 (2023)
PMID: 38003383

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-515809 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 29.02 KDa
Source organism: Blastococcus saxobsidens
Expression system: Escherichia coli
UniProt:
  • Canonical: H6RQF3 (Residues: 1-281; Coverage: 100%)
Gene name: BLASA_4517
Sequence domains: Amino-transferase class IV

Ligands and Environments

2 bound ligands:
Ligand PLP 1 x PLP
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3121
Unit cell:
a: 105.935Å b: 105.935Å c: 51.324Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.171 0.192
Expression system: Escherichia coli

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