8po8

X-ray diffraction
2.52Å resolution

Structure of Escherichia coli HrpA in complex with ADP and oligonucleotide poly(dC)11 forming an i-motif

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-270664 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
ATP-dependent RNA helicase HrpA Chains: A, B
Molecule details ›
Chains: A, B
Length: 758 amino acids
Theoretical weight: 86.31 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P43329 (Residues: 1-758; Coverage: 58%)
Gene names: JW5905, b1413, hrpA
Sequence domains:
DNA (5'-D(P*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 nucleotides
Theoretical weight: 3.14 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 80.26Å b: 105.973Å c: 177.552Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.243
Expression systems:
  • Escherichia coli
  • Not provided