› 8por

X-ray diffraction
1.85Å resolution

Crystal structure of wolbachia leucyl-tRNA synthetase editing domain bound to cmpd6-AMP adduct

Released:
DOI: 10.2210/pdb8por/pdb
PDB entry 8por coloured by chain, front view.
PDB entry 8por coloured by chain, side view.
PDB entry 8por coloured by chain, top view.
Source organism: Wolbachia endosymbiont strain TRS of Brugia malayi
Primary publication:
Targeting a microbiota Wolbachian aminoacyl-tRNA synthetase to block its pathogenic host.
Hoffmann G, Lukarska M, Clare RH, Masters EKG, Johnston KL, Ford L, Turner JD, Ward SA, Taylor MJ, Jensen MR, Palencia A
OpenAccess logo Sci Adv 10 eado1453 (2024)
PMID: 38985862

Function and Biology Details

Reaction catalysed: 
ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274767 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 179 amino acids
Theoretical weight: 20.01 KDa
Source organism: Wolbachia endosymbiont strain TRS of Brugia malayi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5GS31 (Residues: 219-418; Coverage: 20%)
Gene names: Wbm0605, leuS
Sequence domains: Leucyl-tRNA synthetase, editing domain

Ligands and Environments

4 bound ligands:
Ligand SO4 1 x SO4
Ligand EYT 1 x EYT
Ligand EDO 1 x EDO
Ligand PE4 1 x PE4
1 modified residue:
Ligand CSS 1 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P6122
Unit cell:
a: 126.63Å b: 126.63Å c: 61.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.148 0.147 0.166
Expression system: Escherichia coli BL21(DE3)

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