› 8pr7

X-ray diffraction
2.76Å resolution

Aurora-A in complex with CEP192 and an inhibitory monobody

Released:
DOI: 10.2210/pdb8pr7/pdb
PDB entry 8pr7 coloured by chain, front view.
PDB entry 8pr7 coloured by chain, side view.
PDB entry 8pr7 coloured by chain, top view.
Source organisms:
Entry authors: Miles JA, Bayliss R

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-247327 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Aurora kinase A Chains: A, D
Molecule details ›
Chains: A, D
Length: 283 amino acids
Theoretical weight: 32.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14965 (Residues: 122-403; Coverage: 70%)
Gene names: AIK, AIRK1, ARK1, AURA, AURKA, AYK1, BTAK, IAK1, STK15, STK6
Sequence domains: Protein kinase domain
Monobody Chains: B, E
Molecule details ›
Chains: B, E
Length: 96 amino acids
Theoretical weight: 10.13 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Centrosomal protein of 192 kDa Chains: C, F
Molecule details ›
Chains: C, F
Length: 69 amino acids
Theoretical weight: 8.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TEP8 (Residues: 468-533; Coverage: 3%)
Gene names: CEP192, KIAA1569, PP8407

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand GOL 1 x GOL
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P43212
Unit cell:
a: 112.58Å b: 112.58Å c: 216.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.256
Expression system: Escherichia coli

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