› 8ptu

Electron Microscopy
2.52Å resolution

2.5 A cryo-EM structure of the in vitro FimD-catalyzed assembly of type 1 pilus rod

Released:
DOI: 10.2210/pdb8ptu/pdb
PDB entry 8ptu coloured by chain, front view.
PDB entry 8ptu coloured by chain, side view.
PDB entry 8ptu coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The assembly platform FimD is required to obtain the most stable quaternary structure of type 1 pili.
Zyla DS, Wiegand T, Bachmann P, Zdanowicz R, Giese C, Meier BH, Waksman G, Hospenthal MK, Glockshuber R
OpenAccess logo Nat Commun 15 3032 (2024)
PMID: 38589417
Related structures: EMD-17878

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137421 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type-1 fimbrial protein, A chain Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 182 amino acids
Theoretical weight: 18.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04128 (Residues: 1-182; Coverage: 100%)
Gene names: JW4277, b4314, fimA, pilA
Sequence domains: Fimbrial protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.52Å
Relevant EMDB volumes: EMD-17878
Expression system: Escherichia coli BL21(DE3)

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