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Unconventional stabilization of the human T-cell leukemia virus type 1 immature Gag lattice.

<div class='caption-title'>Cryo-ET of immature HTLV-1 Gag VLPs</div>
<div class='caption-title'>Structural model of the immature HTLV-1 CA-NTD interactions</div>
<div class='caption-title'>Increased assembly and aggregation propensity of CA-NTD</div>
Obr M, Percipalle M, Chernikova D, Yang H, Thader A, Pinke G, Porley D, Mansky LM, Dick RA, Schur FK
OpenAccess logo bioRxiv (2023)
Related entries: 8pu6, 8pu7, 8pu8, 8pu9, 8pua, 8pub, 8puc, 8pue, 8puf, 8pug, 8puh

Cited: 1 times
EuropePMC logo PMID: 37546793

Abstract

Human T-cell leukemia virus type 1 (HTLV-1) has an atypical immature particle morphology compared to other retroviruses. This indicates that these particles are formed in a way that is unique. Here we report the results of cryo-electron tomography (cryo-ET) studies of HTLV-1 virus-like particles (VLPs) assembled in vitro , as well as derived from cells. This work shows that HTLV-1 employs an unconventional mechanism of Gag-Gag interactions to form the immature viral lattice. Analysis of high-resolution structural information from immature CA tubular arrays reveals that the primary stabilizing component in HTLV-1 is CA-NTD. Mutagenesis and biophysical analysis support this observation. This distinguishes HTLV-1 from other retroviruses, in which the stabilization is provided primarily by the CA-CTD. These results are the first to provide structural details of the quaternary arrangement of Gag for an immature deltaretrovirus, and this helps explain why HTLV-1 particles are morphologically distinct.

Articles - 8pud mentioned but not cited (1)

  1. research-article Unconventional stabilization of the human T-cell leukemia virus type 1 immature Gag lattice. Obr M, Percipalle M, Chernikova D, Yang H, Thader A, Pinke G, Porley D, Mansky LM, Dick RA, Schur FK. bioRxiv 2023.07.24.548988 (2023)




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