› 8pyx

X-ray diffraction
2.02Å resolution

Amide bond synthetase from Streptomyces hindustanus K492H mutant in complex with Adenosine

Released:
DOI: 10.2210/pdb8pyx/pdb
PDB entry 8pyx coloured by chain, front view.
PDB entry 8pyx coloured by chain, side view.
PDB entry 8pyx coloured by chain, top view.
Source organism: Streptoalloteichus hindustanus
Primary publication:
Broad Spectrum Enantioselective Amide Bond Synthetase from Streptoalloteichus hindustanus.
Tang Q, Petchey M, Rowlinson B, Burden TJ, Fairlamb IJS, Grogan G
ACS Catal 14 1021-1029 (2024)
PMID: 38269041

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-268949 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty-acyl-CoA synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 508 amino acids
Theoretical weight: 54.45 KDa
Source organism: Streptoalloteichus hindustanus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1M5ABR5 (Residues: 1-508; Coverage: 100%)
Gene name: SAMN05444320_10350
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand ADN 2 x ADN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 82.167Å b: 96.159Å c: 87.323Å
α: 90° β: 117.74° γ: 90°
R-values:
R R work R free
0.226 0.224 0.262
Expression system: Escherichia coli

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