› 8q0n

Electron Microscopy
4.2Å resolution

HACE1 in complex with RAC1 Q61L

Released:
DOI: 10.2210/pdb8q0n/pdb
PDB entry 8q0n coloured by chain, front view.
PDB entry 8q0n coloured by chain, side view.
PDB entry 8q0n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural mechanisms of autoinhibition and substrate recognition by the ubiquitin ligase HACE1.
Düring J, Wolter M, Toplak JJ, Torres C, Dybkov O, Fokkens TJ, Bohnsack KE, Urlaub H, Steinchen W, Dienemann C, Lorenz S
OpenAccess logo Nat Struct Mol Biol (2024)
PMID: 38332367
Related structures: EMD-18056

Function and Biology Details

Reactions catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238756 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase HACE1 Chain: B
Molecule details ›
Chain: B
Length: 889 amino acids
Theoretical weight: 99.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8IYU2 (Residues: 22-909; Coverage: 98%)
Gene names: HACE1, KIAA1320
Sequence domains:
Ras-related C3 botulinum toxin substrate 1 Chain: C
Molecule details ›
Chain: C
Length: 213 amino acids
Theoretical weight: 23.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P63000 (Residues: 1-192; Coverage: 100%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family

Ligands and Environments

2 bound ligands:
Ligand 04E 1 x 04E
Ligand GTP 1 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-18056
Expression system: Escherichia coli BL21

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