› 8q26

X-ray diffraction
1.9Å resolution

HsNMT1 in complex with both MyrCoA and GNCFSKPR inhibitor peptide

Released:
DOI: 10.2210/pdb8q26/pdb
PDB entry 8q26 coloured by chain, front view.
PDB entry 8q26 coloured by chain, side view.
PDB entry 8q26 coloured by chain, top view.
Source organisms:
Entry authors: Dian C, Giglione C, Meinnel T

Function and Biology Details

Reaction catalysed: 
Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-250244 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glycylpeptide N-tetradecanoyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 401 amino acids
Theoretical weight: 46.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30419 (Residues: 99-496; Coverage: 80%)
Gene names: NMT, NMT1
Sequence domains:
MYR-GLY-ASN-CYS-PHE-SER-LYS-PRO-ARG Chains: E, F
Molecule details ›
Chains: E, F
Length: 8 amino acids
Theoretical weight: 910 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments


Cofactor: Ligand COA 2 x COA
3 bound ligands:
Ligand CL 2 x CL
Ligand MG 2 x MG
Ligand MYR 2 x MYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21212
Unit cell:
a: 79.22Å b: 177.87Å c: 57.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.161 0.206
Expression systems:
  • Escherichia coli
  • Not provided

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