› 8q2p

X-ray diffraction
1.9Å resolution

Structure of the membrane integral lipoprotein N-acyltransferase Lnt from E. coli by using Se-MAG for the the lipid cubic phase crystallization

Released:
DOI: 10.2210/pdb8q2p/pdb
PDB entry 8q2p coloured by chain, front view.
PDB entry 8q2p coloured by chain, side view.
PDB entry 8q2p coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Se-MAG Is a Convenient Additive for Experimental Phasing and Structure Determination of Membrane Proteins Crystallised by the Lipid Cubic Phase (In Meso) Method
Boland C, Huang CY, Shanker Kaki S, Wang M, Olieric V, Caffrey M
Crystals (2023)

Function and Biology Details

Reaction catalysed: 
A phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine = a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150121 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apolipoprotein N-acyltransferase Chain: A
Molecule details ›
Chain: A
Length: 510 amino acids
Theoretical weight: 56.82 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P23930 (Residues: 1-510; Coverage: 100%)
Gene names: JW0654, b0657, cutE, lnt
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand IRY 15 x IRY
Ligand OLC 4 x OLC
Ligand GOL 6 x GOL
Ligand PG5 1 x PG5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 156.61Å b: 48.79Å c: 75.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.224
Expression system: Escherichia coli

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