› 8q3b

Electron Microscopy
2.69Å resolution

The closed state of the ASFV apo-RNA polymerase

Released:
DOI: 10.2210/pdb8q3b/pdb
PDB entry 8q3b coloured by chain, front view.
PDB entry 8q3b coloured by chain, side view.
PDB entry 8q3b coloured by chain, top view.
Source organism: African swine fever virus BA71V
Primary publication:
Structure of the recombinant RNA polymerase from African Swine Fever Virus.
Pilotto S, Sýkora M, Cackett G, Dulson C, Werner F
OpenAccess logo Nat Commun 15 1606 (2024)
PMID: 38383525
Related structures: EMD-18120

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-239933 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
DNA-directed RNA polymerase RPB1 homolog Chain: A
Molecule details ›
Chain: A
Length: 1450 amino acids
Theoretical weight: 163.94 KDa
Source organism: African swine fever virus BA71V
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P42486 (Residues: 1-1450; Coverage: 100%)
Gene names: Ba71V-99, NP1450L
Sequence domains:
DNA-directed RNA polymerase RPB2 homolog Chain: B
Molecule details ›
Chain: B
Length: 1243 amino acids
Theoretical weight: 140.12 KDa
Source organism: African swine fever virus BA71V
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P42487 (Residues: 1-1242; Coverage: 100%)
Gene names: Ba71V-053, EP1242L
Sequence domains:
DNA-directed RNA polymerase RPB3-11 homolog Chain: C
Molecule details ›
Chain: C
Length: 359 amino acids
Theoretical weight: 41.35 KDa
Source organism: African swine fever virus BA71V
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q65184 (Residues: 1-359; Coverage: 100%)
Gene names: Ba71V-113, H359L
Sequence domains: RNA polymerase Rpb3/Rpb11 dimerisation domain
DNA-directed RNA polymerase RPB7 homolog Chain: D
Molecule details ›
Chain: D
Length: 339 amino acids
Theoretical weight: 38.79 KDa
Source organism: African swine fever virus BA71V
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q89907 (Residues: 1-339; Coverage: 100%)
Gene names: Ba71V-105, D339L
DNA-directed RNA polymerase RPB5 homolog Chain: E
Molecule details ›
Chain: E
Length: 205 amino acids
Theoretical weight: 23.69 KDa
Source organism: African swine fever virus BA71V
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q65181 (Residues: 1-205; Coverage: 100%)
Gene names: Ba71V-108, D205R
Sequence domains: RNA polymerase Rpb5, C-terminal domain
DNA-directed RNA polymerase RPB6 homolog Chain: F
Molecule details ›
Chain: F
Length: 147 amino acids
Theoretical weight: 16.7 KDa
Source organism: African swine fever virus BA71V
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P42484 (Residues: 1-147; Coverage: 100%)
Gene names: Ba71V-069, C147L
Sequence domains: RNA polymerase Rpb6
pC122R Chain: I
Molecule details ›
Chain: I
Length: 105 amino acids
Theoretical weight: 11.83 KDa
Source organism: African swine fever virus BA71V
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q65157 (Residues: 1-105; Coverage: 100%)
Gene names: Ba71V-065, C122R
DNA-directed RNA polymerase RPB10 homolog Chain: J
Molecule details ›
Chain: J
Length: 80 amino acids
Theoretical weight: 9.1 KDa
Source organism: African swine fever virus BA71V
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P42488 (Residues: 1-80; Coverage: 100%)
Gene names: Ba71V-95, CP80R
Sequence domains: RNA polymerases N / 8 kDa subunit

Ligands and Environments

2 bound ligands:
Ligand ZN 6 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.69Å
Relevant EMDB volumes: EMD-18120
Expression system: Trichoplusia ni

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