› 8q3j

X-ray diffraction
2.5Å resolution

Crystal structure of mIL-38 in complex with a neutralizing Fab e04 fragment

Released:
DOI: 10.2210/pdb8q3j/pdb
PDB entry 8q3j coloured by chain, front view.
PDB entry 8q3j coloured by chain, side view.
PDB entry 8q3j coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Neutralizing IL-38 activates γδ T cell-dependent antitumor immunity and sensitizes for chemotherapy.
da Silva P, Mora J, You X, Wiechmann S, Putyrski M, Garcia-Pardo J, Kannt A, Ernst A, Bruene B, Weigert A
J Immunother Cancer 12 (2024)
PMID: 39209451

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-250372 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-1 family member 10 Chains: A, D
Molecule details ›
Chains: A, D
Length: 152 amino acids
Theoretical weight: 17.1 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8R459 (Residues: 1-152; Coverage: 100%)
Gene name: Il1f10
Sequence domains: Interleukin-1 / 18
Fab e04 Light Chain (e04 LC) Chains: B, E
Molecule details ›
Chains: B, E
Length: 223 amino acids
Theoretical weight: 24.6 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Fab e04 Heavy Chain (e04 HC) Chains: C, F
Molecule details ›
Chains: C, F
Length: 241 amino acids
Theoretical weight: 25.65 KDa
Source organism: Mus musculus
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P21
Unit cell:
a: 73.8Å b: 66.94Å c: 129.05Å
α: 90° β: 104.727° γ: 90°
R-values:
R R work R free
0.233 0.227 0.283
Expression systems:
  • Escherichia coli BL21(DE3)
  • Homo sapiens

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