› 8q41

X-ray diffraction
2.38Å resolution

Crystal structure of Can2 (E341A) bound to cA4 and TTTAAA ssDNA

Released:
DOI: 10.2210/pdb8q41/pdb
PDB entry 8q41 coloured by chain, front view.
PDB entry 8q41 coloured by chain, side view.
PDB entry 8q41 coloured by chain, top view.
Source organisms:
Primary publication:
Substrate selectivity and catalytic activation of the type III CRISPR ancillary nuclease Can2.
Jungfer K, Sigg A, Jinek M
OpenAccess logo Nucleic Acids Res (2023)
PMID: 38033326

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235659 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
DUF1887 family protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 439 amino acids
Theoretical weight: 51.34 KDa
Source organism: Thermoanaerobacter brockii subsp. finnii Ako-1
Expression system: Escherichia coli
UniProt:
  • Canonical: E8URK0 (Residues: 1-437; Coverage: 100%)
Gene name: Thebr_0943
DNA (5'-D(*TP*TP*AP*AP*A)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 6 nucleotides
Theoretical weight: 1.81 KDa
Source organism: Thermoanaerobacter brockii subsp. finnii Ako-1
Expression system: Not provided
Cyclic tetraadenosine monophosphate (cA4) Chain: X
Molecule details ›
Chain: X
Length: 4 nucleotides
Theoretical weight: 1.27 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MN 3 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 55.009Å b: 79.322Å c: 95.43Å
α: 90° β: 94.85° γ: 90°
R-values:
R R work R free
0.189 0.186 0.235
Expression systems:
  • Escherichia coli
  • Not provided

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