› 8q5t

X-ray diffraction
2.31Å resolution

Nitrogenase Fe protein from Methanothermococcus thermolithotrophicus, monoclinic crystalline form at 2.31-A resolution

Released:
DOI: 10.2210/pdb8q5t/pdb
PDB entry 8q5t coloured by chain, front view.
PDB entry 8q5t coloured by chain, side view.
PDB entry 8q5t coloured by chain, top view.
Source organism: Methanothermococcus thermolithotrophicus DSM 2095
Primary publication:
Structural comparison of (hyper-)thermophilic nitrogenase reductases from three marine Methanococcales.
Maslać N, Cadoux C, Bolte P, Murken F, Gu W, Milton RD, Wagner T
FEBS J (2024)
PMID: 38696373

Function and Biology Details

Reaction catalysed: 
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-541090 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogenase iron protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 284 amino acids
Theoretical weight: 31.27 KDa
Source organism: Methanothermococcus thermolithotrophicus DSM 2095
UniProt:
  • Canonical: P25767 (Residues: 1-284; Coverage: 100%)
Gene name: nifH1
Sequence domains: 4Fe-4S iron sulfur cluster binding proteins, NifH/frxC family

Ligands and Environments

2 bound ligands:
Ligand GOL 5 x GOL
Ligand SF4 2 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 52.821Å b: 83.984Å c: 116.598Å
α: 90° β: 91.14° γ: 90°
R-values:
R R work R free
0.196 0.194 0.235

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