› 8q69

X-ray diffraction
1.96Å resolution

Crystal structure of HsRNMT complexed with inhibitor DDD1060606

Released:
DOI: 10.2210/pdb8q69/pdb
Model geometry
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PDB entry 8q69 coloured by chain, front view.
PDB entry 8q69 coloured by chain, side view.
PDB entry 8q69 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Characterisation of RNA guanine-7 methyltransferase (RNMT) using a small molecule approach.
Pearson LA, Petit AP, Mendoza Martinez C, Bellany F, Lin D, Niven S, Swift R, Eadsforth T, Fyfe P, Paul M, Postis V, Hu X, Cowling VH, Gray DW
Biochem J 482 (2025)
PMID: 39869500

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128838 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mRNA cap guanine-N(7) methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 276 amino acids
Theoretical weight: 32.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43148 (Residues: 165-415, 456-476; Coverage: 57%)
  • Best match: O43148-2 (Residues: 165-415, 456-473)
Gene names: KIAA0398, RNMT
Sequence domains: mRNA (guanine-N(7))-methyltransferase domain

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
3 bound ligands:
Ligand K7R 2 x K7R
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 39.195Å b: 84.574Å c: 81.966Å
α: 90° β: 99.03° γ: 90°
R-values:
R R work R free
0.244 0.242 0.289
Expression system: Escherichia coli BL21(DE3)

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