› 8q6x

X-ray diffraction
1.91Å resolution

Crystal structure of Cytochrome P450 GymB5 from Streptomyces katrae

Released:
DOI: 10.2210/pdb8q6x/pdb
PDB entry 8q6x coloured by chain, front view.
PDB entry 8q6x coloured by chain, side view.
PDB entry 8q6x coloured by chain, top view.
Source organism: Streptomyces katrae
Primary publication:
Intramolecular Coupling and Nucleobase Transfer - How Cytochrome P450 Enzymes GymBx Establish Their Chemoselectivity
Freytag J, Martin A, Muller JC, Kelm T, Stehle T, Li SM, Zocher G
ChemCatChem 16 (2024)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246147 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chains: A, B
Molecule details ›
Chains: A, B
Length: 410 amino acids
Theoretical weight: 45.07 KDa
Source organism: Streptomyces katrae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0F4JF04 (Residues: 1-393; Coverage: 100%)
Gene name: VR44_17910
Sequence domains: Cytochrome P450

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 63.166Å b: 95.544Å c: 81.459Å
α: 90° β: 109.22° γ: 90°
R-values:
R R work R free
0.171 0.17 0.208
Expression system: Escherichia coli BL21(DE3)

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