› 8qaf

X-ray diffraction
2Å resolution

X-ray crystal structure of a de novo designed single-chain antiparallel 8-helix coiled-coil alpha-helical barrel, sc-apCC-8

Released:
DOI: 10.2210/pdb8qaf/pdb
PDB entry 8qaf coloured by chain, front view.
PDB entry 8qaf coloured by chain, side view.
PDB entry 8qaf coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Rationally seeded computational protein design of ɑ-helical barrels.
Albanese KI, Petrenas R, Pirro F, Naudin EA, Borucu U, Dawson WM, Scott DA, Leggett GJ, Weiner OD, Oliver TAA, Woolfson DN
Nat Chem Biol (2024)
PMID: 38902458

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246665 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
sc-apCC-8 Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 9.62 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P4212
Unit cell:
a: 50.002Å b: 50.002Å c: 57.249Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.249 0.252
Expression system: Escherichia coli

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