8qca

Electron Microscopy
2.84Å resolution

CryoEM structure of a S. Cerevisiae Ski2387 complex in the closed state bound to RNA

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-235804 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Antiviral helicase SKI2 Chain: A
Molecule details ›
Chain: A
Length: 1287 amino acids
Theoretical weight: 146.26 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P35207 (Residues: 1-1287; Coverage: 100%)
Gene names: L8084.17, SKI2, YLR398C
Sequence domains:
Superkiller protein 3 Chain: B
Molecule details ›
Chain: B
Length: 1436 amino acids
Theoretical weight: 164.28 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P17883 (Residues: 1-1432; Coverage: 100%)
Gene names: P9677.7, SKI3, YPR189W
Sequence domains: Tetratricopeptide repeat
Antiviral protein SKI8 Chains: C, D
Molecule details ›
Chains: C, D
Length: 397 amino acids
Theoretical weight: 44.28 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q02793 (Residues: 1-397; Coverage: 100%)
Gene names: REC103, SKI8, YGL213C
Sequence domains: WD domain, G-beta repeat
Superkiller protein 7 Chain: E
Molecule details ›
Chain: E
Length: 250 amino acids
Theoretical weight: 28.35 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08491 (Residues: 1-235; Coverage: 32%)
Gene names: SKI7, YOR076C, YOR29-27
RNA (5'-R(P*UP*UP*UP*U)-3') Chain: X
Molecule details ›
Chain: X
Length: 30 nucleotides
Theoretical weight: 9.14 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.84Å
Relevant EMDB volumes: EMD-18326
Expression systems:
  • Trichoplusia ni
  • Escherichia coli