› 8qcl

X-ray diffraction
1.84Å resolution

A carbohydrate esterase family 15 (CE15) glucuronoyl esterase from Phocaeicola vulgatus ATCC 8482

Released:
DOI: 10.2210/pdb8qcl/pdb
PDB entry 8qcl coloured by chain, front view.
PDB entry 8qcl coloured by chain, side view.
PDB entry 8qcl coloured by chain, top view.
Source organism: Phocaeicola vulgatus ATCC 8482
Primary publication:
Polysaccharide utilization loci from Bacteroidota encode CE15 enzymes with possible roles in cleaving pectin-lignin bonds.
Seveso A, Mazurkewich S, Banerjee S, Poulsen J-CN, Lo Leggio L, Larsbrink J
OpenAccess logo Appl Environ Microbiol e0176823 (2024)
PMID: 38179933

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative acetyl xylan esterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 426 amino acids
Theoretical weight: 48.72 KDa
Source organism: Phocaeicola vulgatus ATCC 8482
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6KWT9 (Residues: 30-427; Coverage: 98%)
Gene name: BVU_0175
Sequence domains: Glucuronyl esterase, fungi

Ligands and Environments

4 bound ligands:
Ligand GCU 2 x GCU
Ligand PEG 1 x PEG
Ligand CL 7 x CL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P21
Unit cell:
a: 74.282Å b: 59.525Å c: 87.452Å
α: 90° β: 101.55° γ: 90°
R-values:
R R work R free
0.175 0.172 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links