› 8qco

X-ray diffraction
1.55Å resolution

E.coli IspE in complex with a ligand (3)

Released:
DOI: 10.2210/pdb8qco/pdb
PDB entry 8qco coloured by chain, front view.
PDB entry 8qco coloured by chain, side view.
PDB entry 8qco coloured by chain, top view.
Source organism: Escherichia coli
Entry author: Hamid R

Function and Biology Details

Reaction catalysed: 
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-251220 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 30.95 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: B7LXC3 (Residues: 1-283; Coverage: 100%)
Gene names: ECIAI1_1229, ispE
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand QBI 2 x QBI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 145.674Å b: 53.162Å c: 91.968Å
α: 90° β: 127.68° γ: 90°
R-values:
R R work R free
0.211 0.209 0.242
Expression system: Escherichia coli

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