› 8qdf

X-ray diffraction
2.2Å resolution

Engineered LmrR with Met-89 replaced by para-boronophenylalanine

Released:
DOI: 10.2210/pdb8qdf/pdb
PDB entry 8qdf coloured by chain, front view.
PDB entry 8qdf coloured by chain, side view.
PDB entry 8qdf coloured by chain, top view.
Source organism: Lactococcus lactis subsp. cremoris MG1363
Primary publication:
Boron catalysis in a designer enzyme.
Longwitz L, Leveson-Gower RB, Rozeboom HJ, Thunnissen AWH, Roelfes G
Nature (2024)
PMID: 38720081

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106936 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription regulator PadR N-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 126 amino acids
Theoretical weight: 14.82 KDa
Source organism: Lactococcus lactis subsp. cremoris MG1363
Expression system: Escherichia coli
UniProt:
  • Canonical: A2RI36 (Residues: 1-116; Coverage: 100%)
Gene name: llmg_0323
Sequence domains: Transcriptional regulator PadR-like family

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 7N8 4 x 7N8

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P21
Unit cell:
a: 60.674Å b: 53.89Å c: 69.034Å
α: 90° β: 95.45° γ: 90°
R-values:
R R work R free
0.21 0.206 0.277
Expression system: Escherichia coli

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