› 8qex

X-ray diffraction
1.9Å resolution

Streptavidin variant with a cobalt catalyst for CH metal-catalyzed hydrogen-atom-transfer (M-HAT)

Released:
DOI: 10.2210/pdb8qex/pdb
PDB entry 8qex coloured by chain, front view.
PDB entry 8qex coloured by chain, side view.
PDB entry 8qex coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
An evolved artificial radical cyclase enables the construction of bicyclic terpenoid scaffolds via an H-atom transfer pathway.
Chen D, Zhang X, Vorobieva AA, Tachibana R, Stein A, Jakob RP, Zou Z, Graf DA, Li A, Maier T, Correia BE, Ward TR
Nat Chem (2024)
PMID: 39030420

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 168 amino acids
Theoretical weight: 17.45 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22629 (Residues: 39-71, 78-183; Coverage: 87%)
Sequence domains: Avidin family

Ligands and Environments

1 bound ligand:
Ligand UFU 2 x UFU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Unit cell:
a: 48.456Å b: 80.534Å c: 78.766Å
α: 90° β: 103.92° γ: 90°
R-values:
R R work R free
0.23 0.229 0.254
Expression system: Escherichia coli BL21(DE3)

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