› 8qfj

X-ray diffraction
1.9Å resolution

Room temperature crystal structure of the Photoactivated Adenylate Cyclase OaPAC after blue light excitation at 2.3 us delay

Released:
DOI: 10.2210/pdb8qfj/pdb
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PDB entry 8qfj coloured by chain, front view.
PDB entry 8qfj coloured by chain, side view.
PDB entry 8qfj coloured by chain, top view.
Source organism: Oscillatoria acuminata PCC 6304
Primary publication:
Light-induced Trpin/Metout switching during BLUF domain activation in ATP-bound photoactivatable adenylate cyclase OaPAC.
Chretien A, Nagel MF, Botha S, de Wijn R, Brings L, Dörner K, Han H, Koliyadu JCP, Letrun R, Round A, Sato T, Schmidt C, Secareanu RC, von Stetten D, Vakili M, Wrona A, Bean R, Mancuso A, Schulz J, Pearson AR, Kottke T, Lorenzen K, Schubert R
J Mol Biol 168439 (2024)
PMID: 38185322

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125708 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Family 3 adenylate cyclase Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 39.29 KDa
Source organism: Oscillatoria acuminata PCC 6304
Expression system: Escherichia coli
UniProt:
  • Canonical: K9TLZ5 (Residues: 1-350; Coverage: 96%)
Gene name: Oscil6304_3613
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand FMN 1 x FMN
Ligand ATP 1 x ATP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: EUROPEAN XFEL BEAMLINE SPB/SFX
Spacegroup: C2221
Unit cell:
a: 54.3Å b: 145.8Å c: 105.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.3 0.297 0.355
Expression system: Escherichia coli

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Citations

1 citations in other articles

ATP-dependent conformational dynamics in a photoactivated adenylate cyclase revealed by fluorescence spectroscopy and small-angle X-ray scattering.
Ujfalusi-Pozsonyi et al. (2024)

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