› 8qfn

X-ray diffraction
1.75Å resolution

Ergothioneine dioxygenase from Thermocatellispora tengchongensis in complex with manganese and in presence of catalase aerobic

Released:
DOI: 10.2210/pdb8qfn/pdb
PDB entry 8qfn coloured by chain, front view.
PDB entry 8qfn coloured by chain, side view.
PDB entry 8qfn coloured by chain, top view.
Source organism: Thermocatellispora tengchongensis
Primary publication:
Enzyme-Catalyzed Oxidative Degradation of Ergothioneine.
Nalivaiko E, Vasseur CM, Seebeck FP
Angew Chem Int Ed Engl e202318445 (2023)
PMID: 38095354

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-237522 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine dioxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 20.7 KDa
Source organism: Thermocatellispora tengchongensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A840P3H4 (Residues: 1-184; Coverage: 100%)
Gene name: HNP84_002159
Sequence domains: Cysteine dioxygenase type I

Ligands and Environments

5 bound ligands:
Ligand ACT 4 x ACT
Ligand PG4 1 x PG4
Ligand EDO 1 x EDO
Ligand MG 1 x MG
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 44.507Å b: 59.209Å c: 134.572Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.225
Expression system: Escherichia coli BL21(DE3)

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