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Cryo-EM structure of SidH from Legionella pneumophila

Electron Microscopy
2.7Å resolution
PDB entry 8qfs coloured by chain, front view.
PDB entry 8qfs coloured by chain, side view.
PDB entry 8qfs coloured by chain, top view.
Source organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Assembly composition:
hetero trimer (preferred)
Entry contents: 2 distinct polypeptide molecules
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: SidH and Elongation factor Tu 2 and RNA
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Multimeric state: hetero trimer
Accessible surface area: 76733.89 Å2
Buried surface area: 9568.27 Å2
Dissociation area: 1,934.08 Å2
Dissociation energy (ΔGdiss): 16.86 kcal/mol
Dissociation entropy (TΔSdiss): 15.59 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-287531

Macromolecules

Elongation factor Tu 2
Chain: C
Length: 394 amino acids
Theoretical weight: 43.37 KDa
Source organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0CE48 (Residues: 1-394; Coverage: 100%)
Gene names: JW3943, b3980, tufB
Pfam:
Elongation factor Tu in PDB entry 8qfs, assembly 1, front view.
Elongation factor Tu in PDB entry 8qfs, assembly 1, side view.
Elongation factor Tu in PDB entry 8qfs, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P0CE48  
139450100150200250300350
 
100200300
UniProt
P0CE48
Chains
Secondary structure
Ligand binding sites
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Sequence conservation
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SidH
Chain: A
Length: 2248 amino acids
Theoretical weight: 255.82 KDa
Source organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Expression system: Legionella pneumophila
UniProt:
  • Canonical: Q5ZRQ1 (Residues: 1-2225; Coverage: 100%)
Gene names: lpg2829, sidH
SidH in PDB entry 8qfs, assembly 1, front view.
SidH in PDB entry 8qfs, assembly 1, side view.
SidH in PDB entry 8qfs, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q5ZRQ1  
122482004006008001,0001,2001,4001,6001,8002,0002,200
 
10002000
UniProt
Q5ZRQ1
Chains
Secondary structure
Interaction interfaces
Sequence conservation
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RNA chain B
Name: tRNA
Representative chains: B
Length: 76 nucleotides
Theoretical weight: 24.62 KDa
tRNA in PDB entry 8qfs, assembly 1, front view.
tRNA in PDB entry 8qfs, assembly 1, side view.
tRNA in PDB entry 8qfs, assembly 1, top view.
17610203040506070
 
204060GCCCGGA*AGCUCAG*CGG*AGAGCAGGGGAUUGAAAAUCCCCGUG*CCUUGG**CGAUUCCGAGUCCGGGCACCA
Chains
Chain B (auth B)
Rfam
Interaction interfaces
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