› 8qh7

X-ray diffraction
1.85Å resolution

Crystal structure of respiratory Complex I subunits NuoEF from Aquifex aeolicus bound to reduced 3-acetylpyridine adenine dinucleotide (without reducing agent)

Released:
DOI: 10.2210/pdb8qh7/pdb
PDB entry 8qh7 coloured by chain, front view.
PDB entry 8qh7 coloured by chain, side view.
PDB entry 8qh7 coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Structures of 3-acetylpyridine adenine dinucleotide and ADP-ribose bound to the electron input module of respiratory complex I.
Wohlwend D, Mérono L, Bucka S, Ritter K, Jessen HJ, Friedrich T
Structure (2024)
PMID: 38503292

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130390 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NADH-quinone oxidoreductase subunit E Chains: A, C
Molecule details ›
Chains: A, C
Length: 160 amino acids
Theoretical weight: 18.57 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli B
UniProt:
  • Canonical: O66842 (Residues: 1-160; Coverage: 100%)
Gene names: aq_574, nuoE
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
NADH-quinone oxidoreductase subunit F Chains: B, D
Molecule details ›
Chains: B, D
Length: 434 amino acids
Theoretical weight: 48.52 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli B
UniProt:
  • Canonical: O66841 (Residues: 1-426; Coverage: 100%)
Gene names: aq_573, nuoF
Sequence domains:

Ligands and Environments

8 bound ligands:
Ligand FES 2 x FES
Ligand SO4 5 x SO4
Ligand NA 9 x NA
Ligand SF4 2 x SF4
Ligand FNR 2 x FNR
Ligand AP0 2 x AP0
Ligand GOL 5 x GOL
Ligand BTB 1 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 63.233Å b: 116.449Å c: 190.047Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.201
Expression system: Escherichia coli B

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