› 8qit

X-ray diffraction
2.9Å resolution

CrPhotLOV1 light state structure 77.5 ms (75-80 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature

Released:
DOI: 10.2210/pdb8qit/pdb
PDB entry 8qit coloured by chain, front view.
PDB entry 8qit coloured by chain, side view.
PDB entry 8qit coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Capturing the blue-light activated state of the Phot-LOV1 domain from Chlamydomonas reinhardtii using time-resolved serial synchrotron crystallography.
Gotthard G, Mous S, Weinert T, Maia RNA, James D, Dworkowski F, Gashi D, Furrer A, Ozerov D, Panepucci E, Wang M, Schertler GFX, Heberle J, Standfuss J, Nogly P
IUCrJ (2024)
PMID: 39037420

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-185298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phototropin Chain: A
Molecule details ›
Chain: A
Length: 138 amino acids
Theoretical weight: 15.34 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8LPD9 (Residues: 16-133; Coverage: 16%)
Gene names: CHLREDRAFT_183965, CHLRE_03g199000v5, PHOT
Sequence domains: PAS domain

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P6522
Unit cell:
a: 121.54Å b: 121.54Å c: 46.18Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.198 0.29
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

The structure of Chlamydomonas LOV1 as revealed by time-resolved serial synchrotron crystallography.
Schmidt M. (2024)

1 mention without citation

Capturing the blue-light activated state of the Phot-LOV1 domain from Chlamydomonas reinhardtii using time-resolved serial synchrotron crystallography.
Gotthard et al. (2024)