› 8qj3

X-ray diffraction
1.9Å resolution

Receptor Sd-Amt1 (OFF-state)

Released:
DOI: 10.2210/pdb8qj3/pdb
PDB entry 8qj3 coloured by chain, front view.
PDB entry 8qj3 coloured by chain, side view.
PDB entry 8qj3 coloured by chain, top view.
Source organism: Shewanella denitrificans OS217
Primary publication:
How sensor Amt-like proteins integrate ammonium signals.
Pflüger T, Gschell M, Zhang L, Shnitsar V, Zabadné AJ, Zierep P, Günther S, Einsle O, Andrade SLA
Sci Adv 10 eadm9441 (2024)
PMID: 38838143

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274513 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ammonium transporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 644 amino acids
Theoretical weight: 70.84 KDa
Source organism: Shewanella denitrificans OS217
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12R70 (Residues: 1-644; Coverage: 100%)
Gene name: Sden_0766
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand LMT 2 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R3
Unit cell:
a: 114.757Å b: 114.757Å c: 278.565Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.162 0.184
Expression system: Escherichia coli BL21(DE3)

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