› 8qj4

X-ray diffraction
1.7Å resolution

Receptor Sd-Amt1 (ON-state)

Released:
DOI: 10.2210/pdb8qj4/pdb
PDB entry 8qj4 coloured by chain, front view.
PDB entry 8qj4 coloured by chain, side view.
PDB entry 8qj4 coloured by chain, top view.
Source organism: Shewanella denitrificans OS217
Primary publication:
How sensor Amt-like proteins integrate ammonium signals.
Pflüger T, Gschell M, Zhang L, Shnitsar V, Zabadné AJ, Zierep P, Günther S, Einsle O, Andrade SLA
Sci Adv 10 eadm9441 (2024)
PMID: 38838143

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245864 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ammonium transporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 644 amino acids
Theoretical weight: 70.84 KDa
Source organism: Shewanella denitrificans OS217
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12R70 (Residues: 1-644; Coverage: 100%)
Gene name: Sden_0766
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CL 4 x CL
Ligand NH4 4 x NH4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: R3
Unit cell:
a: 115.098Å b: 115.098Å c: 275.718Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.151 0.15 0.166
Expression system: Escherichia coli BL21(DE3)

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