› 8qjo

X-ray diffraction
1.85Å resolution

SmNuc1 nuclease from Stenotrophomonas maltophilia in complex with guanosine-5'-monophosphate

Released:
DOI: 10.2210/pdb8qjo/pdb
PDB entry 8qjo coloured by chain, front view.
PDB entry 8qjo coloured by chain, side view.
PDB entry 8qjo coloured by chain, top view.
Source organism: Stenotrophomonas maltophilia
Primary publication:
Substrate preference, RNA binding and active site versatility of Stenotrophomonas maltophilia nuclease SmNuc1, explained by a structural study.
Adámková K, Trundová M, Kovaľ T, Husťáková B, Kolenko P, Dušková J, Skálová T, Dohnálek J
FEBS J (2024)
PMID: 39361520

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-251776 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S1/P1 Nuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 254 amino acids
Theoretical weight: 28.35 KDa
Source organism: Stenotrophomonas maltophilia
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

9 bound ligands:
Ligand ZN 6 x ZN
Ligand PO4 2 x PO4
Ligand 5GP 2 x 5GP
Ligand GOL 2 x GOL
Ligand PEG 2 x PEG
Ligand SO4 19 x SO4
Ligand PGE 1 x PGE
Ligand NA 1 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 43.178Å b: 73.068Å c: 81.607Å
α: 90° β: 105.324° γ: 90°
R-values:
R R work R free
0.172 0.171 0.209
Expression system: Escherichia coli BL21(DE3)

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