› 8qlm

X-ray diffraction
1.66Å resolution

Crystal structure of the pneumococcal Substrate-binding protein AliB in complex with Peptide 3

Released:
DOI: 10.2210/pdb8qlm/pdb
PDB entry 8qlm coloured by chain, front view.
PDB entry 8qlm coloured by chain, side view.
PDB entry 8qlm coloured by chain, top view.
Source organisms:
Primary publication:
Molecular and structural basis of oligopeptide recognition by the Ami transporter system in pneumococci.
Alcorlo M, Abdullah MR, Steil L, Sotomayor F, López-de Oro L, de Castro S, Velázquez S, Kohler TP, Jiménez E, Medina A, Usón I, Keller LE, Bradshaw JL, McDaniel LS, Camarasa MJ, Völker U, Hammerschmidt S, Hermoso JA
OpenAccess logo PLoS Pathog 20 e1011883 (2024)
PMID: 38838057

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-531749 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Oligopeptide-binding protein AliB Chains: A, B
Molecule details ›
Chains: A, B
Length: 626 amino acids
Theoretical weight: 70.02 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A4G1 (Residues: 27-652; Coverage: 100%)
Gene names: aliB, spr1382
Sequence domains: Bacterial extracellular solute-binding proteins, family 5 Middle
PRO-ILE-VAL-GLY-GLY-HIS-GLU-GLY-ALA-GLY-VAL Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 amino acids
Theoretical weight: 993 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P21
Unit cell:
a: 64.204Å b: 108.837Å c: 86.643Å
α: 90° β: 100.05° γ: 90°
R-values:
R R work R free
0.168 0.166 0.205
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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