› 8qmj

X-ray diffraction
0.98Å resolution

Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant H124 soaked with hexa-mannuronic acid

Released:
DOI: 10.2210/pdb8qmj/pdb
PDB entry 8qmj coloured by chain, front view.
PDB entry 8qmj coloured by chain, side view.
PDB entry 8qmj coloured by chain, top view.
Source organism: Paradendryphiella salina
Entry author: Wilkens C

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-498629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alginate lyase 2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 25.52 KDa
Source organism: Paradendryphiella salina
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A7I9C8Z1 (Residues: 17-240; Coverage: 100%)
Gene name: PsAlg7C
Sequence domains: Alginate lyase

Ligands and Environments

Carbohydrate polymer : NEW Components: BEM, MAV
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: C2
Unit cell:
a: 77.35Å b: 38.75Å c: 75.01Å
α: 90° β: 116.9° γ: 90°
R-values:
R R work R free
0.134 0.134 0.157
Expression system: Komagataella pastoris

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