› 8qnr

X-ray diffraction
2.3Å resolution

Crystal structure of ancestral L-galactono-1,4-lactone dehydrogenase: G413N variant in complex with L-gulono-1,4-lactone

Released:
DOI: 10.2210/pdb8qnr/pdb
PDB entry 8qnr coloured by chain, front view.
PDB entry 8qnr coloured by chain, side view.
PDB entry 8qnr coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structure, mechanism, and evolution of the last step in vitamin C biosynthesis.
Boverio A, Jamil N, Mannucci B, Mascotti ML, Fraaije MW, Mattevi A
Nat Commun 15 4158 (2024)
PMID: 38755143

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243785 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-galactono-1,4-lactone dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 511 amino acids
Theoretical weight: 57.31 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand FAD 1 x FAD
Ligand X8L 1 x X8L
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 57.28Å b: 70.026Å c: 69.631Å
α: 90° β: 102.16° γ: 90°
R-values:
R R work R free
0.207 0.203 0.283
Expression system: Escherichia coli

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