› 8qot

Electron Microscopy
3.2Å resolution

Structure of the mu opioid receptor bound to the antagonist nanobody NbE

Released:
DOI: 10.2210/pdb8qot/pdb
PDB entry 8qot coloured by chain, front view.
PDB entry 8qot coloured by chain, side view.
PDB entry 8qot coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis of μ-Opioid Receptor-Targeting by a Nanobody Antagonist.
Yu J, Kumar A, Zhang X, Martin C, Raia P, Koehl A, Laeremans T, Steyaert J, Manglik A, Ballet S, Boland A, Stoeber M
bioRxiv (2023)
PMID: 38106026
Related structures: EMD-18541

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-237600 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Mu-type opioid receptor Chain: A
Molecule details ›
Chain: A
Length: 482 amino acids
Theoretical weight: 53.99 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P42866 (Residues: 2-51, 52-358, 359-398; Coverage: 100%)
Gene names: Mor, Oprm, Oprm1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Nanobody E (NbE) Chain: B
Molecule details ›
Chain: B
Length: 171 amino acids
Theoretical weight: 18.46 KDa
Source organism: Lama glama
Expression system: Escherichia coli
NabFab HC Chain: H
Molecule details ›
Chain: H
Length: 262 amino acids
Theoretical weight: 28.22 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
NabFab LC Chain: L
Molecule details ›
Chain: L
Length: 238 amino acids
Theoretical weight: 25.79 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Anti-Fab Nanobody Chain: K
Molecule details ›
Chain: K
Length: 159 amino acids
Theoretical weight: 17.41 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-18541
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

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