› 8qpp

Electron Microscopy
3.4Å resolution

Bacillus subtilis MutS2-collided disome complex (stalled 70S)

Released:
DOI: 10.2210/pdb8qpp/pdb
PDB entry 8qpp coloured by chain, front view.
PDB entry 8qpp coloured by chain, side view.
PDB entry 8qpp coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
B. subtilis MutS2 splits stalled ribosomes into subunits without mRNA cleavage.
Park EN, Mackens-Kiani T, Berhane R, Esser H, Erdenebat C, Burroughs AM, Berninghausen O, Aravind L, Beckmann R, Green R, Buskirk AR
EMBO J (2023)
PMID: 38177497
Related structures: EMD-18558

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 53-mer (preferred)
PDBe Complex ID:
PDB-CPX-240971 (preferred)
Entry contents:
48 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (53 distinct):
Large ribosomal subunit protein bL32 Chain: 0
Molecule details ›
Chain: 0
Length: 59 amino acids
Theoretical weight: 6.75 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A3A5I502 (Residues: 1-59; Coverage: 100%)
Gene names: BJQ93_03756, FZC70_03495, J5227_17405, KS08_07655, QL281_20730, rpmF
Sequence domains: Ribosomal L32p protein family
Large ribosomal subunit protein bL33 Chain: 1
Molecule details ›
Chain: 1
Length: 48 amino acids
Theoretical weight: 5.79 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A3A5I267 (Residues: 1-48; Coverage: 98%)
Gene names: BJQ93_02949, FZC70_04480, J5227_01080, KS08_12575, QL281_02905, rpmG, rpmGA
Sequence domains: Ribosomal protein L33
Large ribosomal subunit protein bL34 Chain: 2
Molecule details ›
Chain: 2
Length: 44 amino acids
Theoretical weight: 5.27 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XCT1 (Residues: 1-44; Coverage: 100%)
Gene names: FZC70_11675, J5227_06475, KS08_20020, QL281_12165, rpmH
Sequence domains: Ribosomal protein L34
Large ribosomal subunit protein bL35 Chain: 3
Molecule details ›
Chain: 3
Length: 66 amino acids
Theoretical weight: 7.6 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XFQ7 (Residues: 1-66; Coverage: 100%)
Gene names: B4122_3558, B4122_3824, BJQ93_01902, FZC70_06350, J5227_03110, QL281_05335, SC09_Contig17orf00530, rpmI
Sequence domains: Ribosomal protein L35
Large ribosomal subunit protein bL36 Chain: 4
Molecule details ›
Chain: 4
Length: 37 amino acids
Theoretical weight: 4.32 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A199WAC7 (Residues: 1-37; Coverage: 100%)
Gene names: H9S96_00690, H9S96_00850, rpmJ
Sequence domains: Ribosomal protein L36
Large ribosomal subunit protein bL31 Chain: 6
Molecule details ›
Chain: 6
Length: 64 amino acids
Theoretical weight: 7.22 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XD11 (Residues: 1-64; Coverage: 97%)
Gene names: B4122_4678, BJQ93_01737, FZC70_07735, J5227_13160, KS08_18470, QL281_10035, SC09_contig4orf01019, rpmE
Sequence domains: Ribosomal protein L31
Small ribosomal subunit protein uS2 Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 28.01 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XFB4 (Residues: 1-246; Coverage: 100%)
Gene names: B4122_2668, BJQ93_01439, J5227_12585, QL281_21440, SC09_Contig19orf01199, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS3 Chain: C
Molecule details ›
Chain: C
Length: 218 amino acids
Theoretical weight: 24.35 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A3N6BZP3 (Residues: 1-218; Coverage: 100%)
Gene names: B4122_1431, FZC70_20035, J5227_18055, rpsC
Sequence domains:
Small ribosomal subunit protein uS4 Chain: D
Molecule details ›
Chain: D
Length: 200 amino acids
Theoretical weight: 22.87 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XDX5 (Residues: 1-200; Coverage: 100%)
Gene names: B4122_1245, BJQ93_02777, J5227_03450, QL281_05750, rpsD
Sequence domains:
Small ribosomal subunit protein uS5 Chain: E
Molecule details ›
Chain: E
Length: 166 amino acids
Theoretical weight: 17.65 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A1A0G5K9 (Residues: 1-166; Coverage: 100%)
Gene names: FZC70_19980, J5227_18000, QL281_12980, rpsE
Sequence domains:
Small ribosomal subunit protein bS6 Chain: F
Molecule details ›
Chain: F
Length: 95 amino acids
Theoretical weight: 11.14 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XCU1 (Residues: 1-95; Coverage: 100%)
Gene names: B4122_1371, BJQ93_00096, J5227_06555, QL281_12085, SC09_Contig28orf00315, rpsF
Sequence domains: Ribosomal protein S6
Small ribosomal subunit protein uS7 Chain: G
Molecule details ›
Chain: G
Length: 156 amino acids
Theoretical weight: 17.92 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063X741 (Residues: 1-156; Coverage: 100%)
Gene names: B4122_1441, BJQ93_00816, J5227_18110, QL281_12870, SC09_Contig26orf00053, rpsG
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein uS8 Chain: H
Molecule details ›
Chain: H
Length: 132 amino acids
Theoretical weight: 14.9 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XCN0 (Residues: 1-132; Coverage: 100%)
Gene names: B4122_1423, BJQ93_00797, FZC70_19995, J5227_18015, QL281_12965, SC09_Contig26orf00075, rpsH
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS9 Chain: I
Molecule details ›
Chain: I
Length: 130 amino acids
Theoretical weight: 14.34 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XCS1 (Residues: 1-130; Coverage: 100%)
Gene names: B4122_1402, BJQ93_00777, J5227_17910, QL281_13070, SC09_Contig26orf00098, rpsI
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS10 Chain: J
Molecule details ›
Chain: J
Length: 102 amino acids
Theoretical weight: 11.69 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: F5HRV5 (Residues: 1-102; Coverage: 100%)
Gene names: B4122_1437, BJQ93_00812, FZC70_20070, J5227_18090, KS08_00665, QL281_12890, SC09_Contig26orf00059, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
Small ribosomal subunit protein uS11 Chain: K
Molecule details ›
Chain: K
Length: 131 amino acids
Theoretical weight: 13.99 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A7U5HS75 (Residues: 1-131; Coverage: 100%)
Gene names: KS08_00805, rpsK
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS12 Chain: L
Molecule details ›
Chain: L
Length: 138 amino acids
Theoretical weight: 15.25 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XCJ2 (Residues: 1-138; Coverage: 100%)
Gene names: B4122_1442, BJQ93_00817, J5227_18115, QL281_12865, rpsL
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS13 Chain: M
Molecule details ›
Chain: M
Length: 121 amino acids
Theoretical weight: 13.82 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063X7B3 (Residues: 1-121; Coverage: 100%)
Gene names: B4122_1411, BJQ93_00786, J5227_17955, QL281_13025, SC09_Contig26orf00088, rpsM
Sequence domains: Ribosomal protein S13/S18
Small ribosomal subunit protein uS14 Chain: N
Molecule details ›
Chain: N
Length: 61 amino acids
Theoretical weight: 7.26 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: F5HRV2 (Residues: 1-61; Coverage: 100%)
Gene names: B4122_1424, BJQ93_00798, FZC70_20000, J5227_18020, KS08_00735, QL281_12960, SC09_Contig26orf00074, rpsN, rpsN1, rpsZ
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS15 Chain: O
Molecule details ›
Chain: O
Length: 89 amino acids
Theoretical weight: 10.6 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A2J0WEG5 (Residues: 1-89; Coverage: 100%)
Gene names: BJQ93_01458, J5227_12490, QL281_21535, rpsO
Sequence domains: Ribosomal protein S15
Small ribosomal subunit protein bS16 Chain: P
Molecule details ›
Chain: P
Length: 90 amino acids
Theoretical weight: 10.15 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XHU4 (Residues: 1-90; Coverage: 100%)
Gene names: B4122_2720, BJQ93_04298, J5227_12840, QL281_21185, SC09_Contig19orf01134, rpsP
Sequence domains: Ribosomal protein S16
Small ribosomal subunit protein uS17 Chain: Q
Molecule details ›
Chain: Q
Length: 87 amino acids
Theoretical weight: 10.22 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XCK9 (Residues: 1-87; Coverage: 100%)
Gene names: B4122_1428, BJQ93_00802, FZC70_20020, J5227_18040, QL281_12940, SC09_Contig26orf00069, rpsQ
Sequence domains: Ribosomal protein S17
Small ribosomal subunit protein bS18 Chain: R
Molecule details ›
Chain: R
Length: 79 amino acids
Theoretical weight: 8.99 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A3A5I3T1 (Residues: 1-79; Coverage: 100%)
Gene names: BJQ93_00094, J5227_06565, QL281_12075, rpsR
Sequence domains: Ribosomal protein S18
Small ribosomal subunit protein uS19 Chain: S
Molecule details ›
Chain: S
Length: 92 amino acids
Theoretical weight: 10.61 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: F5HRU0 (Residues: 1-92; Coverage: 100%)
Gene names: BJQ93_00807, J5227_18065, KS08_00690, QL281_12915, rpsS
Sequence domains: Ribosomal protein S19
Small ribosomal subunit protein bS20 Chain: T
Molecule details ›
Chain: T
Length: 88 amino acids
Theoretical weight: 9.62 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A086DNG8 (Residues: 1-88; Coverage: 100%)
Gene names: B4122_2452, B4122_4163, BJQ93_02238, J5227_01400, QL281_03235, SC09_Contig24orf00826, rpsT
Sequence domains: Ribosomal protein S20
Endonuclease MutS2 Chain: W
Molecule details ›
Chain: W
Length: 785 amino acids
Theoretical weight: 87.53 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
Large ribosomal subunit protein uL2 Chain: Z
Molecule details ›
Chain: Z
Length: 275 amino acids
Theoretical weight: 30.07 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XCB6 (Residues: 2-276; Coverage: 99%)
Gene names: B4122_1433, BJQ93_00808, J5227_18070, QL281_12910, SC09_Contig26orf00064, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: a
Molecule details ›
Chain: a
Length: 207 amino acids
Theoretical weight: 22.46 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063X745 (Residues: 2-208; Coverage: 99%)
Gene names: B4122_1436, BJQ93_00811, J5227_18085, QL281_12895, SC09_Contig26orf00060, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: b
Molecule details ›
Chain: b
Length: 205 amino acids
Theoretical weight: 22.22 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063X8U6 (Residues: 2-206; Coverage: 99%)
Gene names: B4122_1435, BJQ93_00810, J5227_18080, QL281_12900, SC09_Contig26orf00061, rplD
Sequence domains: Ribosomal protein L4/L1 family
Large ribosomal subunit protein uL5 Chain: c
Molecule details ›
Chain: c
Length: 178 amino acids
Theoretical weight: 20.05 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XB64 (Residues: 2-179; Coverage: 99%)
Gene names: B4122_1425, BJQ93_00799, J5227_18025, QL281_12955, rplE
Sequence domains:
Large ribosomal subunit protein uL6 Chain: d
Molecule details ›
Chain: d
Length: 175 amino acids
Theoretical weight: 19.12 KDa
Source organism: Bacillus subtilis
Large ribosomal subunit protein uL13 Chain: e
Molecule details ›
Chain: e
Length: 142 amino acids
Theoretical weight: 16.02 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XCH0 (Residues: 4-145; Coverage: 98%)
Gene names: B4122_1403, BJQ93_00778, J5227_17915, QL281_13065, rplM
Sequence domains: Ribosomal protein L13
Large ribosomal subunit protein uL14 Chain: f
Molecule details ›
Chain: f
Length: 122 amino acids
Theoretical weight: 13.18 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063X754 (Residues: 1-122; Coverage: 100%)
Gene names: B4122_1427, J5227_18035, SC09_Contig26orf00070, rplN
Sequence domains: Ribosomal protein L14p/L23e
Large ribosomal subunit protein uL15 Chain: i
Molecule details ›
Chain: i
Length: 146 amino acids
Theoretical weight: 15.41 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XCP1 (Residues: 1-146; Coverage: 100%)
Gene names: B4122_1418, BJQ93_00792, J5227_17990, QL281_12990, rplO
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Large ribosomal subunit protein uL16 Chain: j
Molecule details ›
Chain: j
Length: 138 amino acids
Theoretical weight: 15.62 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XB41 (Residues: 1-138; Coverage: 96%)
Gene names: B4122_1430, FZC70_20030, J5227_18050, SC09_Contig26orf00067, rplP
Sequence domains: Ribosomal protein L16p/L10e
Large ribosomal subunit protein bL17 Chain: k
Molecule details ›
Chain: k
Length: 119 amino acids
Theoretical weight: 13.64 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XCG5 (Residues: 2-120; Coverage: 99%)
Gene names: B4122_1408, BJQ93_00783, FZC70_19920, J5227_17940, QL281_13040, SC09_Contig26orf00091, rplQ
Sequence domains: Ribosomal protein L17
Large ribosomal subunit protein uL18 Chain: l
Molecule details ›
Chain: l
Length: 120 amino acids
Theoretical weight: 12.99 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: F5HRS9 (Residues: 1-120; Coverage: 100%)
Gene names: B4122_1421, BJQ93_00795, J5227_18005, QL281_12975, SC09_Contig26orf00077, rplR
Sequence domains: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Large ribosomal subunit protein bL19 Chain: m
Molecule details ›
Chain: m
Length: 115 amino acids
Theoretical weight: 13.42 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XHU6 (Residues: 1-115; Coverage: 100%)
Gene names: B4122_2715, BJQ93_04303, J5227_12815, QL281_21210, SC09_Contig19orf01139, rplS
Sequence domains: Ribosomal protein L19
Large ribosomal subunit protein bL20 Chain: n
Molecule details ›
Chain: n
Length: 117 amino acids
Theoretical weight: 13.41 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XFC1 (Residues: 2-118; Coverage: 98%)
Gene names: B4122_3557, B4122_3823, BJQ93_01903, J5227_03105, QL281_05330, SC09_Contig17orf00528, rplT
Sequence domains: Ribosomal protein L20
Large ribosomal subunit protein bL21 Chain: o
Molecule details ›
Chain: o
Length: 101 amino acids
Theoretical weight: 11.16 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XIS2 (Residues: 2-102; Coverage: 99%)
Gene names: B4122_3686, BJQ93_01994, J5227_02315, QL281_04595, SC09_Contig17orf00255, rplU
Sequence domains: Ribosomal prokaryotic L21 protein
Large ribosomal subunit protein uL22 Chain: r
Molecule details ›
Chain: r
Length: 109 amino acids
Theoretical weight: 12.04 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: F5HRT3 (Residues: 2-110; Coverage: 97%)
Gene names: B4122_1432, BJQ93_00806, KS08_00695, QL281_12920, SC09_Contig26orf00065, rplV
Sequence domains: Ribosomal protein L22p/L17e
Large ribosomal subunit protein uL23 Chain: s
Molecule details ›
Chain: s
Length: 93 amino acids
Theoretical weight: 10.78 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XB36 (Residues: 1-93; Coverage: 98%)
Gene names: B4122_1434, BJQ93_00809, KS08_00680, QL281_12905, SC09_Contig26orf00062, rplW
Sequence domains: Ribosomal protein L23
Large ribosomal subunit protein uL24 Chain: t
Molecule details ›
Chain: t
Length: 101 amino acids
Theoretical weight: 10.92 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: F5HRS7 (Residues: 1-101; Coverage: 98%)
Gene names: B4122_1426, BJQ93_00800, FZC70_20010, J5227_18030, QL281_12950, SC09_Contig26orf00072, rplX
Sequence domains:
Large ribosomal subunit protein bL27 Chain: u
Molecule details ›
Chain: u
Length: 82 amino acids
Theoretical weight: 8.96 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XF22 (Residues: 11-92; Coverage: 87%)
Gene names: B4122_3684, BJQ93_01996, J5227_02305, QL281_04585, SC09_Contig17orf00253, rpmA
Sequence domains: Ribosomal L27 protein
Large ribosomal subunit protein bL28 Chain: v
Molecule details ›
Chain: v
Length: 58 amino acids
Theoretical weight: 6.37 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XHT6 (Residues: 3-60; Coverage: 94%)
Gene names: B4122_2737, BJQ93_04280, FZC70_03125, J5227_12925, QL281_21100, SC09_Contig19orf01114, rpmB
Sequence domains: Ribosomal L28 family
Large ribosomal subunit protein uL29 Chain: w
Molecule details ›
Chain: w
Length: 65 amino acids
Theoretical weight: 7.6 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: F5HRV8 (Residues: 1-65; Coverage: 99%)
Gene names: B4122_1429, BJQ93_00803, FZC70_20025, J5227_18045, KS08_00710, QL281_12935, SC09_Contig26orf00068, rpmC
Sequence domains: Ribosomal L29 protein
Large ribosomal subunit protein uL30 Chain: x
Molecule details ›
Chain: x
Length: 58 amino acids
Theoretical weight: 6.52 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: F5HRU6 (Residues: 2-59; Coverage: 98%)
Gene names: B4122_1419, BJQ93_00793, FZC70_19975, J5227_17995, KS08_00760, QL281_12985, SC09_Contig26orf00079, rpmD
Sequence domains: Ribosomal protein L30p/L7e
Large ribosomal subunit protein bL9 Chain: 9
Molecule details ›
Chain: 9
Length: 149 amino acids
Theoretical weight: 16.38 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: A0A063XEI0 (Residues: 1-149; Coverage: 100%)
Gene names: B4122_1335, J5227_06725, SC09_Contig28orf00268, rplI
Sequence domains:
tRNA (77-MER) Chain: U
Molecule details ›
Chain: U
Length: 77 nucleotides
Theoretical weight: 24.82 KDa
Sequence domains: tRNA
mRNA (33-MER) Chain: V
Molecule details ›
Chain: V
Length: 33 nucleotides
Theoretical weight: 10.59 KDa
5S rRNA (112-MER) Chain: Y
Molecule details ›
Chain: Y
Length: 112 nucleotides
Theoretical weight: 36.16 KDa
Sequence domains: 5S ribosomal RNA
23S ribosomal RNA Chain: X
Molecule details ›
Chain: X
Length: 2928 nucleotides
Theoretical weight: 949.65 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
16S rRNA (1533-MER) Chain: A
Molecule details ›
Chain: A
Length: 1533 nucleotides
Theoretical weight: 496.85 KDa
Sequence domains: Bacterial small subunit ribosomal RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-18558
Expression system: Escherichia coli

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