› 8qpu

Electron Microscopy
5.2Å resolution

Release Complex: BAM bound EspP and Extended SurA

Released:
DOI: 10.2210/pdb8qpu/pdb
PDB entry 8qpu coloured by chain, front view.
PDB entry 8qpu coloured by chain, side view.
PDB entry 8qpu coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Outer membrane protein assembly mediated by BAM-SurA complexes.
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA
Nat Commun 15 7612 (2024)
PMID: 39218969
Related structures: EMD-18562

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-272563 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 790 amino acids
Theoretical weight: 88.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A940 (Residues: 21-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamB Chain: B
Molecule details ›
Chain: B
Length: 373 amino acids
Theoretical weight: 39.88 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P77774 (Residues: 20-392; Coverage: 100%)
Gene names: JW2496, b2512, bamB, yfgL
Sequence domains:
Outer membrane protein assembly factor BamD Chain: D
Outer membrane protein assembly factor BamE Chain: E
Chaperone SurA Chain: F
Outer membrane protein assembly factor BamC Chain: C
Autotransporter protein EspP translocator Chain: P

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.2Å
Relevant EMDB volumes: EMD-18562
Expression system: Escherichia coli

Quick links

Experimental raw data

Links to raw experimental data available for this entry are listed below

Electron microscopy data related to PDB entry 8qpu found at the Electron Microscopy Public Image Archive (EMPIAR)

Data DOI: 10.6019/EMPIAR-11941
Datasets: 1
Total size: 2000Gb
EMPIAR thumbnail