› 8qpu

Electron Microscopy
5.2Å resolution

Release Complex: BAM bound EspP and Extended SurA

Released:
DOI: 10.2210/pdb8qpu/pdb
PDB entry 8qpu coloured by chain, front view.
PDB entry 8qpu coloured by chain, side view.
PDB entry 8qpu coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Outer membrane protein assembly mediated by BAM-SurA complexes.
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA
Nat Commun 15 7612 (2024)
PMID: 39218969
Related structures: EMD-18562

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-272563 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 790 amino acids
Theoretical weight: 88.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A940 (Residues: 21-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamB Chain: B
Molecule details ›
Chain: B
Length: 373 amino acids
Theoretical weight: 39.88 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P77774 (Residues: 20-392; Coverage: 100%)
Gene names: JW2496, b2512, bamB, yfgL
Sequence domains:
Outer membrane protein assembly factor BamD Chain: D
Molecule details ›
Chain: D
Length: 226 amino acids
Theoretical weight: 25.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AC02 (Residues: 20-245; Coverage: 100%)
Gene names: JW2577, b2595, bamD, yfiO
Sequence domains: Outer membrane lipoprotein
Outer membrane protein assembly factor BamE Chain: E
Molecule details ›
Chain: E
Length: 104 amino acids
Theoretical weight: 11.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A937 (Residues: 20-113; Coverage: 100%)
Gene names: JW2598, b2617, bamE, smpA
Sequence domains: SmpA / OmlA family
Chaperone SurA Chain: F
Molecule details ›
Chain: F
Length: 450 amino acids
Theoretical weight: 49.36 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABZ6 (Residues: 21-428; Coverage: 100%)
Gene names: JW0052, b0053, surA
Sequence domains:
Outer membrane protein assembly factor BamC Chain: C
Molecule details ›
Chain: C
Length: 320 amino acids
Theoretical weight: 34.4 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A903 (Residues: 25-344; Coverage: 100%)
Gene names: JW2462, b2477, bamC, dapX, nlpB
Sequence domains: NlpB/DapX lipoprotein
Autotransporter protein EspP translocator Chain: P
Molecule details ›
Chain: P
Length: 364 amino acids
Theoretical weight: 39.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7BSW5 (Residues: 948-984, 985-1300; Coverage: 28%)
Gene names: ECO57PM78, L7020, espP
Sequence domains: Autotransporter beta-domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.2Å
Relevant EMDB volumes: EMD-18562
Expression system: Escherichia coli

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