› 8qq1

X-ray diffraction
1.94Å resolution

SpNOX dehydrogenase domain, mutant F397W in complex with Flavin adenine dinucleotide (FAD)

Released:
DOI: 10.2210/pdb8qq1/pdb
PDB entry 8qq1 coloured by chain, front view.
PDB entry 8qq1 coloured by chain, side view.
PDB entry 8qq1 coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
X-ray structure and enzymatic study of a bacterial NADPH oxidase highlight the activation mechanism of eukaryotic NOX.
Petit-Hartlein I, Vermot A, Thepaut M, Humm AS, Dupeux F, Dupuy J, Chaptal V, Marquez JA, Smith SME, Fieschi F
OpenAccess logo Elife 13 (2024)
PMID: 38640072

Function and Biology Details

Reaction catalysed: 
2 reduced [2Fe-2S] ferredoxin + NAD(+) + H(+) = 2 oxidized [2Fe-2S] ferredoxin + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269349 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding FR-type domain-containing protein Chains: C, F, G
Molecule details ›
Chains: C, F, G
Length: 221 amino acids
Theoretical weight: 25.64 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A4J2B4U9 (Residues: 176-396; Coverage: 56%)
Gene names: SAMEA2341614_01195, ndoR
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 3 x FAD
1 bound ligand:
Ligand BR 26 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P41212
Unit cell:
a: 104.622Å b: 104.622Å c: 142.688Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.225
Expression system: Escherichia coli

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