› 8qq8

X-ray diffraction
2Å resolution

Crystal Structure of F420-dependent Methylene-Tetrahydromethanopterin Reductase Mutant E6Q from Methanocaldococcus Jannaschii

Released:
DOI: 10.2210/pdb8qq8/pdb
PDB entry 8qq8 coloured by chain, front view.
PDB entry 8qq8 coloured by chain, side view.
PDB entry 8qq8 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Mutational and structural studies of (βα)8-barrel fold methylene-tetrahydropterin reductases utilizing a common catalytic mechanism.
Gehl M, Demmer U, Ermler U, Shima S
OpenAccess logo Protein Sci 33 e5018 (2024)
PMID: 38747406

Function and Biology Details

Reaction catalysed: 
5-methyltetrahydromethanopterin + oxidized coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246998 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5,10-methylenetetrahydromethanopterin reductase Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 35.39 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58929 (Residues: 1-331; Coverage: 100%)
Gene names: MJ1534, mer
Sequence domains: Luciferase-like monooxygenase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: I4122
Unit cell:
a: 98Å b: 98Å c: 200.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.235
Expression system: Escherichia coli

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